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Why is the Ames test important?

The Ames test is one of the most common tests for mutagens. It enables the screening of many chemicals, rapidly and inexpensively. Those few chemicals that appear to be mutagenic by the Ames test are then further tested on animals to assess their ability to cause cancer.

Is Ames test in vivo?

bacterial point mutation test (the Ames test), a chromosomal aberrations test in mammalian cells in vitro, and an in vivo (intact animals) test.

Who discovered Ames test?

Bruce Ames

What is the role of liver enzymes in the Ames test?

If the liver enzymes convert the agent to a mutagen, the Ames test will detect it, and it will be labeled as a promutagenic agent. The Ames test is widely used by the pharmaceutical industry to test drugs prior to using them in clinical trials.

Are agents that are mutagenic in humans necessarily carcinogenic?

Mutagens are not necessarily carcinogens, and vice versa. Sodium azide for example may be mutagenic (and highly toxic), but it has not been shown to be carcinogenic.

What is characteristic of the colonies that appear on the plates in the Ames test?

What is characteristic of the colonies that appear on the plates in the Ames test? They are genetically identical to the original Salmonella strain.

How are the results reported in the Ames database reported?

How are the results reported in the Ames Database reported? Results shown with chemical name, Salmonella, TD 50, Rat Tumor Sites (Male, Female) 2. TD is the dose that causes 50% if the rats to develop tumors by the end of their normal lifespans. What is the range of TD in the Ames database?

Which one of the following is not an example of a point mutation?

Which of the following is not a point mutation? Explanation: Point mutation concerns a single base. Thus substitution, which includes transversion, and insertion or deletion, falls within point mutation. On the other hand, translocation involves a stretch of bases so it is not a point mutation.

What is the definition of a mutagen?

(MYOO-tuh-jen) Anything that causes a mutation (a change in the DNA of a cell). DNA changes caused by mutagens may harm cells and cause certain diseases, such as cancer. Examples of mutagens include radioactive substances, x-rays, ultraviolet radiation, and certain chemicals.

What triggers mutation?

Mutations arise spontaneously at low frequency owing to the chemical instability of purine and pyrimidine bases and to errors during DNA replication. Natural exposure of an organism to certain environmental factors, such as ultraviolet light and chemical carcinogens (e.g., aflatoxin B1), also can cause mutations.